黃曲霉素總量ELISA試劑盒(檢飼料/糧食類，靈敏度0.04ppb,一步法)
一、原理

本試劑盒采用間接競爭ELISA方法，在微孔條上預包被偶聯抗原，樣本中的殘留物黃曲素將和微孔條上預包被的偶聯抗原競爭抗黃曲毒素抗體，加入酶標記物后，用TMB底物顯色，樣本吸光值與其所含殘留物黃曲霉素的含量成負相關，與標準曲線比較即可得出相應殘留物黃曲霉素的含量。

二、試劑盒特性
u  試劑盒靈敏度：   0.04ppb
u  孵育溫度：       25℃
u  孵育時間：       30min～15min
u  樣本檢測下限
飼  料·················································2 ppb
花  生················································ 2 ppb
大  米················································ 1.6 ppb
玉  米················································  2 ppb
u  交叉反應率
黃曲霉素B1 ················································  100%
黃曲霉素B2 ················································  223%
黃曲霉素G1················································  78.4%
黃曲霉素G2 ················································  125%
u  樣本回收率
飼料、大米、玉米、花生 ··················· 100±30%

三、試劑盒組成
1 微量測試孔 每條8孔，一板12條
2 標準液×6瓶
（1ml/瓶） 0ppb 0.04ppb
  0.12ppb 0.36ppb
  1.08ppb 3.24ppb
3 酶標記物 7ml 紅色帽
4 抗體工作液 7ml 藍色帽
5 底物A液 7ml 白色帽
6 底物B液 7ml 黑色帽
7 終止液 7ml 黃色帽
8 20X濃縮洗滌液 40ml 白色帽
9 2X濃縮復溶液 50ml 透明帽

四、樣本前處理及實驗分析步驟
   詳見產品說明書。

五、儲藏條件和保質期
1、 儲藏條件：試劑盒于2～8℃保存，不要冷凍。
2、 保 質 期：該產品有效期為1年，生產日期見包裝盒。